Journal: Biomolecules & Therapeutics

Article Title: Aromadendrin Inhibits Lipopolysaccharide-Induced Inflammation in BEAS-2B Cells and Lungs of Mice

doi: 10.4062/biomolther.2024.022

Figure Lengend Snippet: List of antibodies

Article Snippet: p-IκBα (2859S) , Cell Signaling (Danvers, MA, USA) , 40 , 1:1000 , Rabbit , Goat anti rabbit-HRP.

Techniques: Molecular Weight

Journal: Experimental and therapeutic medicine

Article Title: Celastrol inhibits migration and invasion through blocking the NF-κB pathway in ovarian cancer cells.

doi: 10.3892/etm.2017.4568

Figure Lengend Snippet: Figure 4. Effect of celastrol on the nuclear factor‑κB pathway. OVCAR‑3 cells were treated with celastrol for 6 h. Cytosolic and nuclear extracts were subjected to western blotting with the indicated antibodies. Cytosolic extract was probed with the antibody against IκBα, with actin as a loading control, while the nuclear compartment was detected by an anti‑p65 antibody, with PARP as a loading control. PARP, poly ADP ribose polymerase; p, phos phorylated.

Article Snippet: Primary antibodies for western blotting were as follows: Antibodies against p-IκBα (Ser 32) (sc-7977), IκBα (sc-203), p65 (sc-109), poly ADP ribose polymerase (PARP) (sc-1562), MMP-2 (sc-53630), MMP-7 (sc-8832) and MMP-9 (sc-21733) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

Techniques: Western Blot, Control

Journal: Journal of dairy science

Article Title: Sodium butyrate reduces bovine mammary epithelial cell inflammatory responses induced by exogenous lipopolysaccharide, by inactivating NF-κB signaling.

doi: 10.3168/jds.2020-18189

Figure Lengend Snippet: Figure 5. Effects of sodium butyrate (SB) on NF-κB signaling pathway. Bovine mammary epithelial cells (MAC-T) were treated with 1 µg/mL LPS for 6 h; with 0.5 mM SB for 18 h; or with 0.5 mM SB for 18 h, followed by a challenge with 1 µg/mL LPS for an additional 6 h. (A) Western blot analysis of p-IκB α, IκB α, p-p65, and p65. (B) Relative protein expression levels of p-IκB α to IκB α. (C) Relative protein expression levels of p-p65 to p65. Data from the control group were used to normalize the data of each treatment group. Comparisons among groups were calculated using a one-way ANOVA with subsequent Bonferroni correction. Data presented are mean ± SEM. Different lowercase letters in bar charts indicate significant differences (P < 0.05).

Article Snippet: Membranes were then incubated with antibodies against phosphorylatd-IκB α (p-IκB α; sc-271980, Santa Cruz Biotechnology; 1:5,000), IκB α (sc-166588, Santa Cruz Biotechnology; 1:1,000), NFκB p65 (sc-8008, Santa Cruz Biotechnology; 1:1,000), phosphorylatd-NF-κB p65 (p-NF-κB p65; sc-166748, Santa Cruz Biotechnology; 1:1,000), acetyl-H3 (8173, Cell Signaling Technology, Danvers, MA; 1:1,000), histone H3 (4499, Cell Signaling Technology; 1:1,000), and GAPDH (ab8245, Abcam, Cambridge, MA; 1:5,000) overnight at 4°C, followed by incubation with horseradish peroxidase-conjugated anti-mouse or anti-rabbit antibody (Boster, Wuhan, China) for 45 min at room temperature.

Techniques: Western Blot, Expressing, Control